Journal: eBioMedicine

Article Title: Exercise-derived peptide protects against pathological cardiac remodeling

doi: 10.1016/j.ebiom.2022.104164

Figure Lengend Snippet: The primers used in this study.

Article Snippet: The concentrations of CCDC80tide in EVs were measured by ELISA (Human CCDC80 ELISA Kit, EK1962, Boster Bio, USA) combined with immunoblotting assay.

Techniques:

Journal: eBioMedicine

Article Title: Exercise-derived peptide protects against pathological cardiac remodeling

doi: 10.1016/j.ebiom.2022.104164

Figure Lengend Snippet: CCDC80 is a potential exerkine involved in cardiovascular pathophysiology . a. Circos plot visualizes the overlap between upregulated gene lists from three databases, and the blue curves link genes that belong to the same enriched GO term. b. Pie charts represent the network of enriched terms in upregulated genes. Each circle node represents an enriched term and is clustered by its identity, where the size of a slice represents the percentage of genes under the term that originated from the corresponding database. c. Heatmap of enriched terms across the three upregulated gene lists, colored by p -values. d. Heatmap of enrichment analysis in DisGeNET, colored by p-values. e. Circos plot displays the overlap between upregulated gene lists, and the purple curves link identical genes. The five genes within the black box represent the genes with significant upregulation throughout the three databases. f. RT-PCR analysis of the expression levels of CCDC80 in various tissues of mice. g, h. After overexpression of PGC-1α in C2C12 cells, the expressions of Pgc-1α (g) and Ccdc80 (h) was determined by qPCR. i, j and k. Eight-week-old male mice were forced to swim 5 days per week for 3 months. Control groups mice were raised normally without swimming training. After complete swimming training, the skeletal muscles of mice were collected for western blot analysis of CCDC80 and PGC-1α ( n= 7). Data are presented as the average of four biological replicates (n = 4) ± SD. Statistical significance was determined using Student's t-test, *** p < 0.001.

Article Snippet: The concentrations of CCDC80tide in EVs were measured by ELISA (Human CCDC80 ELISA Kit, EK1962, Boster Bio, USA) combined with immunoblotting assay.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Over Expression, Control, Muscles, Western Blot

Journal: eBioMedicine

Article Title: Exercise-derived peptide protects against pathological cardiac remodeling

doi: 10.1016/j.ebiom.2022.104164

Figure Lengend Snippet: CCDC80 generates a peptide encapsulated in EVs in response to PGC-1α overexpression . a. The cell lysates and culture supernatants collected from vehicle- or Flag-PGC-1α-overexpressing C2C12 cells (treated or not with BFA, 1 μg/mL, 16 h) were prepared and analyzed for CCDC80 protein levels by WB. N= 3 b. The supernatants of CCDC80-EGFP-overexpressing C2C12 cells were harvested and filtered through a sterile 0.22-μm filter and then added to H9c2 cells. After 12-h incubation, the cell fluorescence was detected. The fluorescent spots indicated by white arrows were supposedly EVs. N= 3 c, d, e. The supernatants of CCDC80-overexpressing C2C12 cells were collected, and EVs were extracted for TEM (c), NTA (d), and EVs markers (e) determinations. N= 3. WCL: whole cell lysates. f. The cell lysates and EVs isolated from the supernatants of vehicle- or CCDC80-HA-overexpressing C2C12 cells were prepared and analyzed for CCDC80 protein levels by WB. g. The schematic diagram of CCDC80tide in CCDC80.

Article Snippet: The concentrations of CCDC80tide in EVs were measured by ELISA (Human CCDC80 ELISA Kit, EK1962, Boster Bio, USA) combined with immunoblotting assay.

Techniques: Over Expression, Sterility, Incubation, Fluorescence, Isolation

Journal: EMBO Reports

Article Title: A novel long intergenic noncoding RNA indispensable for the cleavage of mouse two‐cell embryos

doi: 10.15252/embr.201642051

Figure Lengend Snippet: In normal two‐cell mouse embryos, LincGET works as a transcription factor, mediating the cis‐regulatory activity of GLKLTRs, and an inhibitor of exon skipping, partially through decreasing hnRNP U, FUBP1, and ILF2 protein levels (up). However, when LincGET is depleted, disordered transcription and disordered RNA splicing would result in disordered ZGA, MAPK signaling inhibition, and exon skipping of some M‐phase‐associated genes, including Cdk1, leading to G2/M block (down).

Article Snippet: CDK1 protein domain prediction by InterPro ( http://www.ebi.ac.uk/interpro/ ).

Techniques: Activity Assay, Inhibition, Blocking Assay

Journal: BMC Genomics

Article Title: Sequencing, annotation, and comparative genome analysis of the gerbil-adapted Helicobacter pylori strain B8

doi: 10.1186/1471-2164-11-335

Figure Lengend Snippet: List of 12 singletons of strain B8, i.e. genes which completely occur outside of the uncovered regions and have neither an 80/80 blastp hit in the B128 proteome nor a complete match with at most 2% differences on the DNA level.

Article Snippet: 12 , HPB8_1618 , hypothetical protein predicted by Glimmer/Critica , , .

Techniques: Expressing, Chemotaxis Assay

Journal: BMC Genomics

Article Title: Sequencing, annotation, and comparative genome analysis of the gerbil-adapted Helicobacter pylori strain B8

doi: 10.1186/1471-2164-11-335

Figure Lengend Snippet: Functional alignment of the three plasmids of the H. pylori strains B8 (pHPB8), P12 (pHPP12), and HPP8 (pHel4) . Homologuous genes are shown in the same color. The cluster of mob -genes (green) and the rep A-gene (yellow) are present in all three plasmids. Genes homologuous to pHPB8_1 (plasmid stabilization system protein) and to pHPB8_2 (conserved hypothetical protein) are present in pHPP12 and pHel4 (red). The microcins mcc C and mcc B are not present in pHPB8. Genes that are not present in the other plasmids are colored white (pHPB8), orange (pHPP12) and light blue (pHel4).

Article Snippet: 12 , HPB8_1618 , hypothetical protein predicted by Glimmer/Critica , , .

Techniques: Functional Assay, Plasmid Preparation